The Center for Proactive Medicine; Paige Adams, FNP, B-C's Facebook Wall 2013-10-22 21:19:13
Photos from Carol Savage's post in MTHFR Gene MutationUNDERSTANDING…LYME WESTERN BLOT… and why it is not reliable.
There are 9 known Borrelia burgdorferi [Bb] genus specific KDA [Kiladaltons] Western Blot antibodies. These are often referred to as BANDS.
Only one of the above Bb genus specie specific BANDS is needed to confirm that there is serologic evidence of EXPOSURE to the Bb spirochete and can confirm a clinical diagnosis of Lyme disease.
CDC [Center for Disease Control] Western Blot IgM antibody surveillance criteria includes only 2 Borrelia burgdorferi spirochete genus specie specific antibodies for IgM 23 and 39 and excludes all the other 7 BANDS.
CDC Western Blot IgG surveillance criteria includes √ BANDS 18, 23, 37, 39, and 93…and excludes BANDS *31, 34, and 83.
30 OspA substrate binding protein; common in European and one California strain.
It does not make sense:
1. to exclude any Borrelia burgdorferi genus specific antibodies in a Lyme Western Blot IgG and to
2. include only two of the antibodies in their IgM form, [ because all the antibodies in IgG form were all once IgM. ]
IgM converts to IgG in about two months unless there is a persisting infection driving a persisting IgM reaction. This is the case with any infection including the Borrelia burgdorferi induced Lyme disease.
The CDC further…WRONGFULLY includes 5 non-specific cross-reacting antibodies in its Western Blot surveillance criteria:
41…flagellar [other bacteria have flagella too] 45
This leads to the possibility of “false positive Lyme Western Blots”
There would be no false positives if only Borrelia burgdorferi genus specific antibodies were considered.
One could have a CDC surveillance positive IgG Lyme Western Blot with the five non-specific antibodies without having any Borrelia burgdorferi genus specie specific antibodies. This does not make sense.
The CDC recommends that the Lyme Western Blot be performed only if there is a positive or equivocal [+/-] Lyme ELISA screening tests. In Dr. Charles Ray Jones’ practice he cared for over 7,000 children with Lyme disease, 30% of which had a CDC positive Lyme Western Blot tests with negative [-] ELISA screening tests. The Lyme ELISA is a poor screening test. An adequate screening test should have false positives
not false negative tests.
Charles Ray Jones, MD
Madison Towers…………….. Phone: 203-772-1123…FAX: 203-772-0682
111 Park St., Suite F
PICTURE: Jemsek Western Blot example
It is curious to note that some of our patients only convert to a laboratory positive after they have received antimicrobial therapy, whether it is oral, intravenous, or a combination of the two
We suspect this phenomenon stems from Bb die-off on therapy, with an ensuing boosted immune response.
As we have stated, fully one-third or more of all patients with active Bb infection will test negative with current methods (far more will be undiagnosed employing Lyme disease illiterate MDs), and so, as much as those of us in Infectious Disease would like a positive or confirmatory laboratory diagnostic report to comfort us, and we will continue to strive for this piece of paper to "soothe our souls", we agree with the CDC that neuroborreliosis remains a …clinical diagnosis.